CURCUMIN REVERSES DICHLORVOS-MEDIATED ERYTHROCYTE TOXICOSIS IN RATS BY ATTENUATING OXIDATIVE STRESS AND DEPLETION OF CELLULAR ACETYLCHOLINESTERASE

Authors

  • A. J. AKAMO 1. Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. 2.Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria.
  • B. A. OLAGUNJU Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. O. ADELEYE Department of Animal Production and Health, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
  • O. A. AKINLOYE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • M. A. AKINSANYA Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria.
  • A. O. OJELABI Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. ALUKO Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. O. FAWIBE Department of Pure and Applied Botany, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
  • O. T. KAYODE Department of Biochemistry, Mountain Top University, Ibafo, Ogun State, Nigeria.
  • O. O AYODELE Department of Biochemistry, Mountain Top University, Ibafo, Ogun State, Nigeria.
  • D. I. AKINLOYE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. T. SOMADE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • S. O. SALAMI Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • A. S. JAMES Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • E. I. UGWOR Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. E. ETENG Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • C. A. MOSES Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • I. O. OPOWOYE Department of Animal Production and Health, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
  • M. I. OLASOJU Department of Veterinary Public Health and Preventive Medicine, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • A. M. ADEOSUN Department of Biochemistry, Lead City University, Ibadan, Nigeria
  • O. O. HASSAN Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • A. O. WILLSONKORO Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • O. S. AYINDE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • A. H. EHINAFE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • I. O. ONIYIDE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • A. O. AJETUNMOBI Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • T. M. ALLI Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • T. E. OLADELE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
  • J. K. AKINTUNDE Clinical Biochemistry and Mechanistic Toxicology Research Cluster, Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

Keywords:

Curcumin, dichlorvos, erythrocyte, oxidative stress, acetylcholinesterase

Abstract

In low- and middle-income countries, including Nigeria, dichlorvos (DDVP) is a prevalent organophosphate insecticide. However, its misuse as a suicide agent and presence in food items endangers the human populace. Curcumin is known to modulate free radicals, antioxidant proteins, and lipid peroxidation. However, its effect on DDVP-induced erythrocyte intoxication remains undocumented in the scientific literature. Herein the influence of curcumin was examined on DDVP-mediated erythrocyte toxicosis in Wistar rats. Forty-two rats were randomly allocated to seven groups of six rats each: normal control, DDVP alone (20 mg/kg/day), DDVP delivered with curcumin (50 and 100 mg/kg/day) or atropine (0.2 mg/kg/day) as a reference drug, and curcumin alone (50 and 100 mg/kg/day). Rats were humanely killed after one week of gavage DDVP treatment and another two weeks of curcumin therapy; blood was collected, and erythrocytes were isolated. Sub-acute administration of curcumin markedly (p<0.05) attenuated DDVP-provoked augmentation in the erythrocyte concentrations of NO and malondialdehyde and the activity of GST.  Curcumin abrogated DDVP-mediated decreased in erythrocyte GSH levels and activities of SOD, catalase, and glutathione peroxidase, acetylcholinesterase. Ultimately, curcumin ameliorated DDVP-mediated erythrocyte toxicosis via anti-oxidative and cholinergic mechanisms.  

 

References

Abu-Taweel, G. M. 2016. Effects of curcumin on the social behavior, blood composition, reproductive hormones in plasma and brain acetylcholinesterase in cadmium intoxicated mice. Saudi Journal of Biological Sciences 23(2): 219 - 228.

Akamo, A. J., Akinloye, D. I., Ugbaja, R. N., Adeleye, O. O., Dosumu, O. A., Eteng, O. E., Antiya, M. C., Amah, G., Ajayi, O. A. and Faseun, S. O. 2021. Naringin prevents cyclophosphamide-induced erythrocytotoxicity in rats by abrogating oxidative stress. Toxicology Reports 8: 1803 - 1813.

Akande, M. G. and Ahmed, U. S. 2017. Taurine abated subacute dichlorvos toxicity. Toxicology Reports 4: 463 - 466.

Akinyemi, A. J., Oboh, G., Fadaka, A. O., Olatunji, B. P. and Akomolafe, S. 2017. Curcumin administration suppresses acetylcholinesterase gene expression in cadmium treated rats. Neurotoxicology 62: 75-79.

Buege, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. In methods in enzymology 52: 302 - 310. Academic Press.

Dwivedi, N., Bhutia, Y. D., Kumar, V., Yadav, P., Kushwaha, P., Swarnkar, H. and Flora, S. J. S. 2010. Effects of combined exposure to dichlorvos and monocrotophos on blood and brain biochemical variables in rats. Human and Experimental Toxicology 29(2): 121-129.

Farkhondeh, T., Mehrpour, O., Forouzanfar, F., Roshanravan, B. and Samarghandian, S. 2020. Oxidative stress and mitochondrial dysfunction in organophosphate pesticide-induced neurotoxicity and its amelioration: a review. Environmental Science and Pollution Research 27: 24799 - 24814.

Forouzanfar, F., Read, M.I., Barreto, G.E. and Sahebkar, A. 2020. Neuroprotective effects of curcumin through autophagy modulation. International Union of Biochemistry and Molecular Biology Life 72(4): 652 - 664.

Habig, W. H., Pabst, M. J., Fleischner, G., Gatmaitan, Z., Arias, I. M. and Jakoby, W. B. 1974. The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. Proceedings of the National Academy of Sciences 71(10): 3879 – 3882.

Hadwan, M. H. and Abed, H. N. 2016. Data supporting the spectrophotometric method for the estimation of catalase activity. Data in Brief 6: 194 – 199.

Kępińska-Pacelik, J. and Biel, W. 2023. Turmeric and curcumin-health-promoting properties in humans versus dogs. International Journal of Molecular Sciences 24(19): 14561.

Leskovac, A. and Petrović, S. 2023. Pesticide use and degradation strategies: food safety, challenges and perspectives. Foods 12(14): 2709.

Marklund, S. and Marklund, G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47(3): 469 - 474.

Messarah, M., Amamra, W., Boumendjel, A., Barkat, L., Bouasla, I., Abdennour, C. and Feki, A. E. 2013. Ameliorating effects of curcumin and vitamin E on diazinon-induced oxidative damage in rat liver and erythrocytes. Toxicology and Industrial Health 29(1): 77 - 88.

Mfaume, H., Pratap, H., Luziga, C. and Urasa, F. 2023. Ameliorative effects of vitamins C and E on haematotoxicity and spleen histopathology induced by dichlorvos insecticide in female wistar rats. Tanzania Journal of Science 49(2): 516 - 527.

Nan, P., Yan, S., Li, L., Chen, J., Du, Q. and Chang, Z. 2015. Toxicity effect of dichlorvos on loach (Misgurnus anguillicaudatus) assessed by micronucleus test, hepatase activity analysis and comet assay. Toxicology and Industrial Health 31(6): 566 - 575.

Nwamba H., O., Achikanu C. E. and Chukwu Ginika, P. 2018. The impact of dichlorvos-pesticide on African catfish (Clarias gariepinus). Oceanography & Fisheries Open Access Journal 8(4): 122 - 126.

Okoroiwu, H. U. and Iwara, I. A. 2018. Dichlorvos toxicity: A public health perspective. Interdisciplinary Toxicology 11(2): 129-137.

Oridupa, O.A. 2020. Knowledge, attitude and perception of related toxicity of pesticide exposure in humans and animals in Ibadan, Nigeria. Sokoto Journal of Veterinary Sciences 18(3): 129 - 136.

Oyeyemi, W. A., Daramola, O. O. O., Akinola, A. O., Idris, A. O. and Aikpitanyi, I. 2020. Hepatic and reproductive toxicity of sub-chronic exposure to dichlorvos and lead acetate on male Wistar rats. Asian Pacific Journal of Reproduction 9(6): 283-290.

Percie du Sert, N., Hurst, V., Ahluwalia, A., Alam, S., Avey, M. T., Baker, M. and Würbel, H. 2020. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. Journal of Cerebral Blood Flow and Metabolism 40(9): 1769 - 1777.

Rahman, I., Kode, A. and Biswas, S.K. 2006. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nature protocols 1(6): 3159 - 3165.

Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G. and Hoekstra, W. 1973. Selenium: biochemical role as a component of glutathione peroxidase. Science 179(4073): 588 - 590.

Sreejayan, X. X. and Rao, M. N. A. 1997. Nitric oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology 49(1): 105 - 107.

Suwarta, F. X., Suryani, C. L., Astuti, N. and Amin, L. 2022. Effects of dietary supplementation of turmeric and black cumin powder on performance and blood parameters of native chickens. Online Journal of Animal and Feed Research 12(6): 390 - 397.

Tietz, N. W. 1995. Clinical guide to laboratory tests. In Clinical guide to laboratory tests pp. 1096 - 1996.

Wellington, D., Mikaelian, I. and Singer, L. 2013. Comparison of ketamine–xylazine and ketamine–dexmedetomidine anesthesia and intraperitoneal tolerance in rats. Journal of the American Association for Laboratory Animal Science 52(4): 481 - 487.

Yadav, P., Jadhav, S., Kumar, V., Kaul, K., Pant, S. and Flora, S. 2012. Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats. Interdisciplinary Toxicology 5(1): 1 – 8.

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Published

2024-12-30

Issue

Vol. 23 No. 1 (2024)

Section

Articles