PREVENTIVE ROLE OF Ficus exasperata N-HEXANE: ETHYL ACETATE (85:15 v/v) FRACTION AGAINST POTASSIUM DICHROMATE INDUCED OXIDATIVE STRESS IN FEMALE WISTAR RATS
Keywords:
Potassium dichromate, Oxidative stress, Ficus exasperata, Intoxication.Abstract
Humans and animals exposed to potassium dichromate develop oxidative stress-associated diseases. Given Ficus exasperata's medicinal properties, this study investigated how Ficus exasperata's n-hexane: ethyl acetate fraction (FR2) can prevent potassium dichromate-induced oxidative stress in female Wistar rats. The study assessed oxidative stress indicators (malondialdehyde (MDA), total antioxidant capacity (TAC) aconitase (ACON) and non-enzymatic antioxidants (total thiols (TSH), vitamin C (Vit C), and vitamin E (Vit E). Thirty female Wistar rats, divided into six groups (n=5), were used for this study. Group A (control) and B were administered olive oil (2 ml/kg b.wt (body weight)) for fourteen days. For an exact number of days, group C (Standard) was administered vitamin E (100 mg/kg b.wt) and omega 3 (72 mg/kg b.wt eicosapentaenoic acid+ 48 mg/kg b.wt docosahexaenoic acid). In comparison, groups D (30 mg/kg b.wt), E (60 mg/kg b.wt), and F (60 mg/kg b.wt) were administered Ficus exasperata n-hexane: ethyl acetate fraction (FR2). The experiment lasted for fourteen days, and potassium dichromate (100 mg/kg b.wt) was administered on the fourteenth day to rats in groups B, C, D, and E. Groups D and E pre-treated with FR2 showed the protective role of Ficus exasperata n-hexane: ethyl acetate fraction against high levels of serum ACON and MDA with concomitant low levels of T.SH, Vit C, Vit E, TAC and tissues (heart, liver, and kidney) ACON when compared to group B (Potassium dichromate intoxicated). This research revealed that the n-hexane: ethyl acetate fraction of Ficus exasperata leaf extract protects female Wistar rats against potassium dichromate-induced oxidative stress.References
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