IDENTIFICATION AND SUSCEPTIBILITY PATTERNS OF SOME SKIN BACTERIA ISOLATES USING VITEK-2 SYSTEM
Keywords:
Vitek-2 system, bacteria; isolates; infections; skin.Abstract
The use of the automated Vitek-2 system as a sensitive tool in the identification and antibiotic susceptibility of microorganisms cannot be overemphasized especially for its fast, efficient and reliable technology. This study evaluated the Vitek-2 system for identification and susceptibility pattern of some isolated bacteria from skin infections. Two hundred and seventy-two skin swab samples from pupils with skin infections in some selected primary schools across the three geopolitical zones of Ogun State were examined for this study. Isolation and comparison of bacteria identification and susceptibility pattern were achieved using standard microbiological techniques, agar disc diffusion method and Vitek-2 system, respectively. Out of the two hundred and seventy-two (272) skin swab samples collected, twenty-eight (28) were identified to specie-level by Vitek-2 system. Staphylococcus aureus, Staphylococcus hominis, Staphylococcus cohnii, Staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus xylosis, Staphylococcus agalactiae and Staphylococcus lugdunensis were the definitive species of Staphylococcus identified. Staphylococcus aureus 13 (46.43%) had the highest prevalence of identity, however, Staphylococcus lugdunensis, Staphylococcus cohnii and Staphylococcus xylosis had the least occurrence of 1(3.57%) each. The isolates were highly susceptible to ciprofloxacin 24(85.71%), followed by gentamycin 21(75%) and nitrofurantoin 20(71.43%). All the isolates were absolutely resistant to benzylpenicillin 28(100%). A significant increase in the zone of inhibition of ciprofloxacin when compared with cefuroxime (F-ratio=9.325, P-value <0.05) was observed using the agar disc diffusion method. The study concluded that the Vitek-2 system is a definitive and comprehensive technique for bacteria identification and antibiotic susceptibility, thus providing rapid and accurate results while saving time and w
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