BEHAVIOURAL IMPLICATIONS OF EXPOSURE TO ULTRAVIOLET AND INFRARED LIGHTS ON WISTAR ALBINO RAT
Keywords:
Behaviour, Ultraviolet light, Infrared light, Wistar albino ratAbstract
The crucial role played by different light wavelengths can evoke different behavioural responses in animals, especially when introduced at night. This study investigated the effects of exposure to near Ultraviolet (UV) and near Infrared (IR) lights on exploratory, memory and antidepressant behaviour of male Wistar albino rat (Rattus norvegicus). Thirty weaned male rats (30.02±5.82 g) were exposed to daylight (6 AM to 6 PM) and 6 hours of artificial lights of varying wavelengths (UVA-365 nm, UVA-396 nm, IRA-850 nm and IRA-940 nm) at night for 90 days. The control groups were exposed to darkness (DRK) and ambient light (AML), respectively. Light treatments and control were replicated five times. Behavioural outcomes were measured using the Open Field Test (OFT), Forced Swim Test (FST) and Novel Object Recognition Test (NOR). The highest immobility time in FST was highest in DRK (110.00±6.33 s) while UVA 365 nm had the lowest immobility time (41.60±23.72 s). In the OFT, rats exposed to ultraviolet A (365 nm) light showed significantly (p < 0.05) higher exploratory and non-depressive behaviour: centre square duration (44.40±10.46 s), grooming duration (110.80±28.05 s), rearing duration (103.40±38.56 s). Rats exposed to UVA (396 nm) had the highest discrimination index for the novel object (0.03) in NOR test. In conclusion, exposure of male Wistar rats to ultraviolet and infrared lights of varying wavelengths had significant impact on the depressive, memory and exploratory behaviour.
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